TRAM1 antibody (C-Term)

Catalog No. ABIN2856617

The Rabbit Polyclonal anti-TRAM1 antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect TRAM1 in samples from Human.

Quick Overview for TRAM1 antibody (C-Term) (ABIN2856617)

Target: TRAM1 (Translocation Associated Membrane Protein 1 (TRAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRAM1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to TRAM1 (translocation associated membrane protein 1)
TRAM1 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRAM1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TRAM1

Target: TRAM1 (Translocation Associated Membrane Protein 1 (TRAM1))

Alternative Name: translocation associated membrane protein 1

Background: This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause.

Cellular Localization: Endoplasmic reticulum membrane, Multi-pass membrane protein

Molecular Weight: 43 kDa

Gene ID: 23471

UniProt: Q15629