Liver Arginase antibody

Catalog No. ABIN2856661

This Rabbit Polyclonal antibody specifically detects Liver Arginase in WB, ELISA, FACS, IHC (p), IF, IP, ICC and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 11+ publications.

Quick Overview for Liver Arginase antibody (ABIN2856661)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-Liver Arginase Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Arginase 1 (arginase, liver)
Arginase 1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human Arginase 1 Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:1000. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.26 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Liver Arginase antibody (ABIN2856661)

Scherwitzl, Opp, Hurtado, Pampeno, Loomis, Kannan, Yu, Meruelo: "Sindbis Virus with Anti-OX40 Overcomes the Immunosuppressive Tumor Microenvironment of Low-Immunogenic Tumors." in: Molecular therapy oncolytics, Vol. 17, pp. 431-447, (2020) (PubMed).

Glass, Masjedi, Dudzinski, Wilson, Duvall, Yull, Giorgio: "Optimizing Mannose "Click" Conjugation to Polymeric Nanoparticles for Targeted siRNA Delivery to Human and Murine Macrophages." in: ACS omega, Vol. 4, Issue 16, pp. 16756-16767, (2019) (PubMed).

Gomez, Baylis, Durgin, Newman, Alencar, Mahan, St Hilaire, Müller, Waisman, Francis, Pinteaux, Randolph, Gram, Owens: "Interleukin-1β has atheroprotective effects in advanced atherosclerotic lesions of mice." in: Nature medicine, Vol. 24, Issue 9, pp. 1418-1429, (2019) (PubMed).

Zhang, Tang, Huang, Zhou, Cui, Weng, Liu, Kim, Lee, Perez-Neut, Ding, Czyz, Hu, Ye, He, Zheng, Shuman, Dai, Ren, Roeder, Becker, Zhao: "Metabolic regulation of gene expression by histone lactylation." in: Nature, Vol. 574, Issue 7779, pp. 575-580, (2019) (PubMed).

Shen, Li, Ma, Tian, Hong, Zhai, Li, Huang, Shi: "IRAK-M alters the polarity of macrophages to facilitate the survival of Mycobacterium tuberculosis." in: BMC microbiology, Vol. 17, Issue 1, pp. 185, (2018) (PubMed).

Zhu, Zhang, Lu, Gao, Cai, Sui, Su, Shen, Xie: "Identification of different macrophage subpopulations with distinct activities in a mouse model of oxygen-induced retinopathy." in: International journal of molecular medicine, Vol. 40, Issue 2, pp. 281-292, (2018) (PubMed).

Wu, Sun, Li, Shen, Zhai, Yu, Chen et al.: "Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory ..." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 36, (2018) (PubMed).

Romano, Parrinello, Vetro, Tibullo, Giallongo, La Cava, Chiarenza, Motta, Caruso, Villari, Tripodo, Cosentino, Ippolito, Consoli, Gallamini, Pileri, Di Raimondo: "The prognostic value of the myeloid-mediated immunosuppression marker Arginase-1 in classic Hodgkin lymphoma." in: Oncotarget, Vol. 7, Issue 41, pp. 67333-67346, (2018) (PubMed).

Carmona-Fontaine, Deforet, Akkari, Thompson, Joyce, Xavier: "Metabolic origins of spatial organization in the tumor microenvironment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 11, pp. 2934-2939, (2017) (PubMed).

Beachley, Wolf, Sadtler, Manda, Jacobs, Blatchley, Bader, Pandey, Pardoll, Elisseeff: "Tissue matrix arrays for high-throughput screening and systems analysis of cell function." in: Nature methods, Vol. 12, Issue 12, pp. 1197-204, (2016) (PubMed).

Harmon, Fronhofer, Keller, Feustel, Zhu, Xu, Avram, Jones, Nagarajan, Lennartz: "Anti-Inflammatory Immune Skewing Is Atheroprotective: Apoe-/-Fc?RIIb-/- Mice Develop Fibrous Carotid Plaques." in: Journal of the American Heart Association, Vol. 3, Issue 6, (2014) (PubMed).

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: arginase 1

Background: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Cellular Localization: Cytoplasm

Molecular Weight: 35 kDa

Gene ID: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin