UBE4B antibody (C-Term)

Catalog No. ABIN2856674

The Rabbit Polyclonal anti-UBE4B antibody has been validated for WB, IHC (p), IF and ICC. It is suitable to detect UBE4B in samples from Human.

Quick Overview for UBE4B antibody (C-Term) (ABIN2856674)

Target: UBE4B (Ubiquitin Conjugation Factor E4 B (UBE4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-UBE4B Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to UBE4B (ubiquitination factor E4B (UFD2 homolog, yeast))
UBE4B antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UBE4B

Target: UBE4B (Ubiquitin Conjugation Factor E4 B (UBE4B))

Alternative Name: ubiquitination factor E4B

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Cellular Localization: Cytoplasm

Molecular Weight: 146 kDa

Gene ID: 10277

UniProt: O95155