PHKA1 antibody (C-Term)

Catalog No. ABIN2856675

This anti-PHKA1 antibody is a Rabbit Polyclonal antibody detecting PHKA1 in WB, IF, IHC (p) and ICC. Suitable for Human.

Quick Overview for PHKA1 antibody (C-Term) (ABIN2856675)

Target: PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-PHKA1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Mouse (96 %), Rat (95 %)

Characteristics: Rabbit Polyclonal antibody to PHKA1 (phosphorylase kinase, alpha 1 (muscle))
PHKA1 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human PHKA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PHKA1

Target: PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))

Alternative Name: PHKA1

Background: The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB, MIM 172490), and gamma (PHKG1, MIM 172470 and PHKG2, MIM 172471) subunits have several isoforms, the delta subunit is calmodulin (CALM1, MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]

Cellular Localization: Cell membrane, Lipid-anchor, Cytoplasmic side

Molecular Weight: 137 kDa

Gene ID: 5255

Pathways: Cellular Glucan Metabolic Process