CDC45 antibody (Center)

Catalog No. ABIN2856678

This anti-CDC45 antibody is a Rabbit Polyclonal antibody detecting CDC45 in WB, IF and ICC. Suitable for Human.

Quick Overview for CDC45 antibody (Center) (ABIN2856678)

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC45 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDC45 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Cow (Bovine)

Cross-Reactivity (Details): Mouse (93 %), Bovine (90 %)

Characteristics: Rabbit polyclonal antibody to CDC45L (CDC45 cell division cycle 45-like (S. cerevisiae))
CDC45L antibody [C1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CDC45L. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Alternative Name: CDC45L

Background: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 66 kDa

Gene ID: 8318

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA