RAG2 antibody (Center)

Catalog No. ABIN2856698

The Rabbit Polyclonal anti-RAG2 antibody is suitable to detect RAG2 in samples from Human and Rat. It has been validated for WB, IF, IHC (p) and ICC.

Quick Overview for RAG2 antibody (Center) (ABIN2856698)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-RAG2 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (85 %), Pig (90 %), Rabbit (91 %), Rat (86 %), Bovine (91 %)

Characteristics: Rabbit Polyclonal antibody to RAG2 (recombination activating gene 2)
RAG2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Molt-4 , rat thymus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.37 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2

Background: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Cellular Localization: Nucleus

Molecular Weight: 59 kDa

Gene ID: 5897

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response