RAG2 antibody (AA 350-527)

Catalog No. ABIN969379

Product Details anti-RAG2 Antibody

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Binding Specificity: AA 350-527

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: purified

Immunogen: Purified recombinant fragment of human RAG2(350-527aa) expressed in E. coli.

Clone: 4D5

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-RAG2 antibody (ABIN969379)

Nagawa, Hirose, Nishizumi, Nishihara, Sakano: "Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38360-8, (2004) (PubMed).

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2 (RAG2 Products)

Background:

Description: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Aliases: RAG-2, RAG2

Molecular Weight: 59 kDa

Gene ID: 5897

HGNC: 5897

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response