FH antibody

Catalog No. ABIN2856710

The Rabbit Polyclonal anti-FH antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect FH in samples from Human. There is 1 publication available.

Quick Overview for FH antibody (ABIN2856710)

Target: FH (Fumarate Hydratase (FH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-FH Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Fumarate hydratase (fumarate hydratase)
Fumarate hydratase antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse liver , rat liver , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.49 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-FH antibody (ABIN2856710)

Miettinen, Felisiak-Golabek, Wasag, Chmara, Wang, Butzow, Lasota: "Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases." in: The American journal of surgical pathology, Vol. 40, Issue 12, pp. 1661-1669, (2017) (PubMed).

Target Details for FH

Target: FH (Fumarate Hydratase (FH))

Alternative Name: fumarate hydratase

Background: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Cellular Localization: Mitochondrion , Cytoplasm

Molecular Weight: 55 kDa

Gene ID: 2271

UniProt: P07954