CCT5 antibody

Catalog No. ABIN2856715

This Rabbit Polyclonal antibody specifically detects CCT5 in WB, IF, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CCT5 antibody (ABIN2856715)

Target: CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-CCT5 Antibody

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to TCP1 epsilon (chaperonin containing TCP1, subunit 5 (epsilon))
TCP1 epsilon antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TCP1 epsilon. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CCT5

Target: CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Alternative Name: chaperonin containing TCP1 subunit 5

Background: This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Cellular Localization: Cytoplasm

Molecular Weight: 60 kDa

Gene ID: 22948

UniProt: P48643