GLDC antibody

Catalog No. ABIN2856747

This anti-GLDC antibody is a Rabbit Polyclonal antibody detecting GLDC in WB, IP, ICC, IF and IHC (p). Suitable for Human.

Quick Overview for GLDC antibody (ABIN2856747)

Target: GLDC (Glycine Dehydrogenase (GLDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLDC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLDC Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Glycine dehydrogenase (glycine dehydrogenase (decarboxylating))
Glycine dehydrogenase antibody [N3C2-2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Glycine dehydrogenase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Validation: Comparison

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.46 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GLDC

Target: GLDC (Glycine Dehydrogenase (GLDC))

Alternative Name: glycine decarboxylase

Background: The enzyme system for cleavage of glycine (glycine cleavage system, GCS, EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE, MIM 605899) may be due to a defect in any one of these enzymes, see MIM 238310, MIM 238330, and MIM 238331.[supplied by OMIM]

Cellular Localization: Mitochondrion

Molecular Weight: 113 kDa

Gene ID: 2731

UniProt: P23378