Aminoacylase 1 antibody

Catalog No. ABIN2856751

This Rabbit Polyclonal antibody specifically detects Aminoacylase 1 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for Aminoacylase 1 antibody (ABIN2856751)

Target: Aminoacylase 1 (ACY1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aminoacylase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Aminoacylase 1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to Aminoacylase 1
Aminoacylase 1 antibody [N2C3-2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Aminoacylase 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Aminoacylase 1

Target: Aminoacylase 1 (ACY1)

Alternative Name: aminoacylase 1

Background: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Cellular Localization: Cytoplasm

Molecular Weight: 46 kDa

Gene ID: 95

UniProt: Q03154