NDUFA5 antibody (full length)

Catalog No. ABIN2856783

The Rabbit Polyclonal anti-NDUFA5 antibody (ABIN2856783) specifically detects NDUFA5 in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for NDUFA5 antibody (full length) (ABIN2856783)

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NDUFA5 Antibody

Binding Specificity: full length

Cross-Reactivity: Chimpanzee

Cross-Reactivity (Details): Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to NDUFA5 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13 kDa)
NDUFA5 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human NDUFA5 Recombinant protein.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: H1299 , HeLa , HepG2 , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDUFA5

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Alternative Name: NDUFA5

Background: The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.

Cellular Localization: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side

Molecular Weight: 13 kDa

Gene ID: 4698