CAMK2D antibody

Catalog No. ABIN2856806

The Rabbit Polyclonal anti-CAMK2D antibody has been validated for WB, IF, IHC (p), ICC and IP. It is suitable to detect CAMK2D in samples from Human. There are 3+ publications available.

Quick Overview for CAMK2D antibody (ABIN2856806)

Target: CAMK2D (Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2D antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-CAMK2D Antibody

Specificity: Cross-reactivity may occur with other CaM kinase II subunits.

Cross-Reactivity: Human, Mouse, Rabbit, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to CaMKII delta (calcium/calmodulin-dependent protein kinase II delta)
CaMKII delta antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CaMKII delta. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549 , H1299 , HCT116 , 293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.62 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CAMK2D antibody (ABIN2856806)

Liu, Zhong, Zhou, Yang, Tan, Li, Gao, Sun, Li, Jin, Cao, Yuan, Liu, Liang, Li, Du, Zhao, Xue, Zhao, Song, Ling, Li: "Alteration of calcium signalling in cardiomyocyte induced by simulated microgravity and hypergravity." in: Cell proliferation, Vol. 53, Issue 3, pp. e12783, (2020) (PubMed).

Li, Liu, Li, Zheng, Xu, Liu, Sun, Li, Ji, Liu, Zhang, Zhao, Du, Liu, Zhong, Sun, Wang, Song, Zhang, Qin, Ling, Wang, Li: "TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling." in: Nature communications, Vol. 10, Issue 1, pp. 1589, (2019) (PubMed).

Xu, Wang, Zhang, Gong, Gutierrez Cortes, Zhu, Yoon, Tian, Wang: "CaMKII induces permeability transition through Drp1 phosphorylation during chronic β-AR stimulation." in: Nature communications, Vol. 7, pp. 13189, (2016) (PubMed).

Target Details for CAMK2D

Target: CAMK2D (Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D))

Alternative Name: calcium/calmodulin dependent protein kinase II delta

Background: The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.

Molecular Weight: 56 kDa

Gene ID: 817

UniProt: Q13557

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration