PDE1A antibody

Catalog No. ABIN2856821

The Rabbit Polyclonal anti-PDE1A antibody is suitable to detect PDE1A in samples from Human. It has been validated for IHC (p) and WB.

Quick Overview for PDE1A antibody (ABIN2856821)

Target: PDE1A (Calcium/calmodulin-Dependent 3',5'-Cyclic Nucleotide Phosphodiesterase 1A (PDE1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details anti-PDE1A Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PDE1A (calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A)
PDE1A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PDE1A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299 , HeLa , mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.74 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PDE1A

Target: PDE1A (Calcium/calmodulin-Dependent 3',5'-Cyclic Nucleotide Phosphodiesterase 1A (PDE1A))

Alternative Name: phosphodiesterase 1A

Background: Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1, MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109], Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM]

Molecular Weight: 61 kDa

Gene ID: 5136

UniProt: P54750