CXCR7 antibody

Catalog No. ABIN2857021

This Rabbit Polyclonal antibody specifically detects CXCR7 in WB, ELISA, FACS, IF, IHC (p), IP, ICC and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 7+ publications.

Quick Overview for CXCR7 antibody (ABIN2857021)

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-CXCR7 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to CXCR7 (C-X-C chemokine receptor type 7)
CXCR7 antibody [C1C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR7. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validatio: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.92 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CXCR7 antibody (ABIN2857021)

Wei, Huang, Hsieh, Lin, Shyu, Chen, Hsieh: "Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling." in: Cell death & disease, Vol. 11, Issue 5, pp. 307, (2020) (PubMed).

Liu, Song, Setroikromo, Chen, Hu, Chen, van der Wekken, Melgert, Timens, van den Berg, Saber, Haisma: "CX Chemokine Receptor 7 Contributes to Survival of KRAS-Mutant Non-Small Cell Lung Cancer upon Loss of Epidermal Growth Factor Receptor." in: Cancers, Vol. 11, Issue 4, (2019) (PubMed).

Xu, Tang, Wang, Liu, Fu, Luo: "CXCR7 promotes melanoma tumorigenesis via Src kinase signaling." in: Cell death & disease, Vol. 10, Issue 3, pp. 191, (2019) (PubMed).

Tam, Ko, Koon, Cheng, Lok, Lau, Leung, Fung, Chan, Lau: "Identification of Target Genes Involved in Wound Healing Angiogenesis of Endothelial Cells with the Treatment of a Chinese 2-Herb Formula." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139342, (2016) (PubMed).

Liu, Carson-Walter, Walter: "Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells." in: PLoS ONE, Vol. 9, Issue 8, pp. e103938, (2015) (PubMed).

Salazar, Muñoz, Kallifatidis, Singh, Jordà, Lokeshwar: "The chemokine receptor CXCR7 interacts with EGFR to promote breast cancer cell proliferation." in: Molecular cancer, Vol. 13, pp. 198, (2014) (PubMed).

Singh, Lokeshwar: "The IL-8-regulated chemokine receptor CXCR7 stimulates EGFR signaling to promote prostate cancer growth." in: Cancer research, Vol. 71, Issue 9, pp. 3268-77, (2011) (PubMed).

Target Details for CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Alternative Name: atypical chemokine receptor 3

Background: This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.

Cellular Localization: Cell membrane

Molecular Weight: 41 kDa

Gene ID: 57007

UniProt: P25106

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling