APOE antibody (C-Term)

Catalog No. ABIN2857040

The Rabbit Polyclonal anti-APOE antibody is suitable to detect APOE in samples from Human. It has been validated for WB, ELISA, IP, ICC, IHC (p), IF and IHC (fro).

Quick Overview for APOE antibody (C-Term) (ABIN2857040)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APOE Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Apolipoprotein E (apolipoprotein E)
Apolipoprotein E antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Conditional medium from differentiated human primary preadipocyte , HepG2 , human plasma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.66 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: apolipoprotein E

Background: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Cellular Localization: Secreted

Molecular Weight: 36 kDa

Gene ID: 348

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism