BAK1 antibody (N-Term)

Catalog No. ABIN2857046

The Rabbit Polyclonal anti-BAK1 antibody has been validated for WB and IHC (p). It is suitable to detect BAK1 in samples from Human.

Quick Overview for BAK1 antibody (N-Term) (ABIN2857046)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAK1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to BAK (BCL2-antagonist/killer 1)
BAK antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BAK. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: BCL2 antagonist/killer 1

Background: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Cellular Localization: Membrane

Molecular Weight: 23 kDa

Gene ID: 578

UniProt: Q16611

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response