LYZ antibody (AA 1-129)

Catalog No. ABIN285848

Product Details anti-LYZ Antibody

Target: LYZ (Lysozyme (LYZ))

Binding Specificity: AA 1-129

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): All forms of lysozyme from chicken. Cross reactivity against Lysozyme from other sources may occur but has not been specifically determined.

Purification: Ion exchange chromatography

Immunogen: Lysozyme antibody was raised in rabbit using full length protein corresponding to amino acids 1-129 of Hen Egg White lysozyme as the immunogen.

Application Details

Application Notes: ELISA: 1:25,000, IP: 1:100, WB: 1:500-1:2,000, IHC: 1:500-1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °Cfor short term storage and at-20 °C for long term storage.

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: Lysozyme (LYZ Products)

Background: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of ß-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme c is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme g. Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.