VRN2 antibody

Catalog No. ABIN7477853

This Mouse Monoclonal antibody specifically detects VRN2 in ELISA and WB. It exhibits reactivity toward Triticum aestivum.

Quick Overview for VRN2 antibody (ABIN7477853)

Target: VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Reactivity: Triticum aestivum

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Product Details anti-VRN2 Antibody

Purpose: Anti-VRN2 | Vernalization protein 2

Cross-Reactivity (Details): Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Triticum monococcum

Purification: Protein G purified

Immunogen: KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Application Details

Application Notes: 1 : 10 000 (ELISA), 1 : 1000 (WB)

Comment:

there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Total IgG. Protein G purified purified from Cell culture supernatant.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for VRN2

Target: VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))

Alternative Name: VRN2

Background:

Background: Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Additional information: VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Molecular Weight: 23.7 kDa