Toxoplasma Gondii P30 antibody

Catalog No. ABIN2859480

The Mouse Monoclonal anti- antibody has been validated for WB, EIA and IF. It is suitable to detect in samples from Toxoplasma gondii.

Quick Overview for Toxoplasma Gondii P30 antibody (ABIN2859480)

Target: Toxoplasma Gondii P30

Reactivity: Toxoplasma gondii

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Clone: 203

Product Details

Purification: Protein A chromatography.

Purity: > 90 %.

Immunogen: Trophozoites lysate

Isotype: IgG2

Application Details

Application Notes: Suitable for use in IFA, ELISA and immunoblotting. A starting range of 1: 10-1: 50 is suggested for IFA and blotting and 1: 20-1: 200 for ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2 containing 0.09 % sodium azide as preservative and no stabilizers.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Toxoplasma Gondii P30

Background: Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Gene ID: 5811

UniProt: Q27298