The Mouse Monoclonal anti-RSV antibody is suitable to detect RSV in samples from Respiratory Syncytial Virus (RSV). It has been validated for IF and EIA.
RSV
Reactivity: Human, Human respiratory syncytial virus A, Virus
IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 488
Application Notes
Suitable for use in indirect IFA and ELISA. A starting range of 1: 10 - 1: 50 is suggested for IFA and 1: 20 - 1: 200 for ELISA. Does not work in Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 and with 0.09 % Sodium azide as preservative. Without stabilizing proteins .
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
RSV
(Respiratory Syncytial Virus (RSV))
Alternative Name
Respiratory Syncytial Virus / RSV
Target Type
Virus
Background
Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.