Bordetella pertussis antibody

Catalog No. ABIN2859505

This Mouse Monoclonal antibody specifically detects Bordetella pertussis in WB, IF and EIA. It exhibits reactivity toward Bordetella pertussis.

Quick Overview for Bordetella pertussis antibody (ABIN2859505)

Target: Bordetella pertussis

Reactivity: Bordetella pertussis

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: BDI500

Product Details

Purification: Protein A chromatography

Purity: > 90 %.

Immunogen: Bordetella pertussis N-lauroyl sarcosine membrane extract

Isotype: IgG3

Application Details

Application Notes: Suitable for use in ELISA, IFA and Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, containing 0.09 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Bordetella pertussis

Target Type: Bacteria

Background: Bordetella pertussis is an aerobic, gram negative coccobacilli. It causes whooping cough (pertussis), an acute respiratory infection marked by severe, spasmodic coughing episodes during the paroxysmal phase. Leukocytosis with lymphocytosis is also common during this phase of the illness. Dangerous complications are bronchopneumonia and acute encephalopathy. It produces a number of virulence factors, including pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, and hemolysin. Agglutinogens and other outer membrane proteins are important antigens.