UVRAG antibody (Biotin)

Catalog No. ABIN2868821

This Rabbit Polyclonal antibody specifically detects UVRAG in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for UVRAG antibody (Biotin) (ABIN2868821)

Target: UVRAG (UV Radiation Resistance Associated Gene (UVRAG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UVRAG antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-UVRAG Antibody (Biotin)

Specificity: Predicted molecular weight at ~78.1 kDa. Observed molecular weights in the 75-90 kDa range.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the mid-protein of human UVRAG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2868821 was sufficient for detection of UVRAG on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for UVRAG

Target: UVRAG (UV Radiation Resistance Associated Gene (UVRAG))

Alternative Name: UVRAG

Background: UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (1). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (2). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (3-4). It is highly expressed in the brain, lung, kidney and liver.

Gene ID: 7405

NCBI Accession: NP_003360

UniProt: Q9P2Y5