Fibronectin antibody (Biotin)

Catalog No. ABIN288224

The Rabbit Polyclonal anti-Fibronectin antibody is suitable to detect Fibronectin in samples from Human. It has been validated for WB, IHC, IP and ELISA.

Quick Overview for Fibronectin antibody (Biotin) (ABIN288224)

Target: Fibronectin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA

Product Details anti-Fibronectin Antibody (Biotin)

Specificity: This antibody is directed against mouse angiopoietin 1 and shows no reativity withmouse angiopoietin 2.

Cross-Reactivity (Details): This antibody reacts with Human Fibronectin. Ths antibody has negligible crossreactivity with Type I,II,III,IV,V,VI Collagens or Laminin1.0 mg/ml

Purification: Affinity chromatography purified

Sterility: Filtered

Immunogen: Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Application Details

Application Notes: ELISA: 1:5,000-1:20,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:500-1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid (sterile filtered) in 0.02 M Potassium Phosphate, 0.12 Sodium Chloride, pH 7.2 with 10 mg/mL BSA and 0.01 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectin is a high-molecular weight (~440 kDa) extracellular matrix glycoprotein that binds to membrane-spanning receptor proteins called integrins. In addition to integrins, fibronectin also binds extracellular matrix components such as collagen, fibrin and heparan sulfate proteoglycans (e.g. syndecans).