Vitronectin antibody (AA 121-133)

Catalog No. ABIN2888249

This Mouse Monoclonal antibody specifically detects Vitronectin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Vitronectin antibody (AA 121-133) (ABIN2888249)

Target: Vitronectin (VTN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vitronectin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: HV2

Product Details anti-Vitronectin Antibody

Binding Specificity: AA 121-133

Specificity: Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). This antibody is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse). The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133. Reactivity: Can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitative vitronectin in human plasma in a sandwich ELISA with clone 6F211. It does not interfere with the binding of any known vitronectin ligands. Also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well.

Purification: Protein A/G purified

Immunogen: Human vitronectin purified from plasma by heparin-affinity chromatography.

Type of Immunogen: Purified protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA (1:32000), WB (1:100)

Usage: Suitable for use in ELISA, Western Blot. ELISA: 1:32000. Western Blot: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Vitronectin

Target: Vitronectin (VTN)

Alternative Name: VTN / Vitronectin

Background: Name/Gene ID: VTN
Family: Matrix Protein

Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

Gene ID: 7448

UniProt: P04004

Pathways: Autophagy, Smooth Muscle Cell Migration