GFP antibody (Green Fluorescent Protein) (HRP)

Catalog No. ABIN289132

Product Details anti-GFP Antibody

Target:

GFP

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to HRP

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Specificity: The monoclonal detects wild type and recombinant GFP. This antibody does notdetect enhanced GFP.

Immunogen: GFP antibody (HRP) was raised in Mouse using The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. as the immunogen.

Clone: 215

Isotype: IgG2a

Target Details for GFP

Target:

GFP

Alternative Name: GFP

Target Type: Tag

Application Details

Application Notes: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % thimerisol. Immunoglobulin and protease free.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.