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GFP antibody (HRP)

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Mouse Monoclonal 215 HRP

Catalog No. ABIN289132

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
166

15

11

9
Aequorea victoria
Host
60

57

36

13

9

5

1

1
Mouse
Compatible Secondaries
Clonality
111

67
Monoclonal
Conjugate
80

11

10

9

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to HRP
Application
143

78

46

45

30

28

25

23

16

13

13

13

13

11

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

The monoclonal detects wild type and recombinant GFP. This antibody does notdetect enhanced GFP.

Immunogen

GFP antibody (HRP) was raised in Mouse using The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. as the immunogen.

Clone

215

Isotype

IgG2a

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Application Notes

ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Format

Lyophilized

Concentration

Lot specific

Buffer

Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % thimerisol. Immunoglobulin and protease free.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)