GFP antibody (Green Fluorescent Protein) (HRP)

Catalog No. ABIN289132

Product Details anti-GFP Antibody

Target: GFP

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Reactivity: Aequorea victoria

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• Aequorea victoria 189
• Tag 7
• All Species 6
• General 6
• Aequorea aequorea 5
• Human 3
• Mouse 2
• Others 2
• Rat 2
• Chemical 1
• Jellyfish 1
• Pontellina plumata 1

Host: Mouse

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• Rabbit 76
• Mouse 73
• Goat 32
• Chicken 16
• Camelid (Camelidae) 12
• Sheep 7
• Rat 3
• Guinea Pig 1

Clonality: Monoclonal

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• Polyclonal 129
• Monoclonal 87

Conjugate: This GFP antibody is conjugated to HRP

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• Un-conjugated 119
• FITC 11
• Biotin 10
• HRP 9
• Alkaline Phosphatase (AP) 5
• Texas Red (TR) 5
• Alexa Fluor 647 3
• GFP tag 3
• APC 2
• Alexa Fluor 488 2
• Alexa Fluor 555 2
• Alexa Fluor 594 2
• Alexa Fluor 680 2
• Alexa Fluor 750 2
• Atto 647N 2
• Cy3 2
• Cy5 2
• DyLight 405 2
• DyLight 488 2
• DyLight 549 2
• DyLight 649 2
• DyLight 680 2
• DyLight 800 2
• TRITC 2
• Abberior STAR 580 1
• Abberior STAR 635P 1
• AbberiorStarRED 1
• Alexa Fluor 350 1
• Atto 488 1
• Atto 542 1
• Atto 565 1
• CF®488A 1
• CF®568 1
• CF®594 1
• CF®633 1
• CF®640R 1
• CF®660R 1
• CF®680 1
• CF®680R 1
• CF®770 1
• Cy5.5 1
• Cy7 1
• CypHer5E 1
• Oyster550 1
• Oyster650 1
• PE 1
• Peroxidase (POD) 1
• Rhodamine 1

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

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• Western Blotting (WB) 153
• ELISA 92
• Immunofluorescence (IF) 47
• Immunohistochemistry (IHC) 45
• Immunoprecipitation (IP) 34
• Immunocytochemistry (ICC) 33
• Flow Cytometry (FACS) 32
• Fluorescence Microscopy (FM) 22
• Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14
• FLISA 12
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10
• Immunohistochemistry (Frozen Sections) (IHC (fro)) 7
• Immunochromatography (IC) 6
• Enzyme Immunoassay (EIA) 5
• Dot Blot (DB) 4
• Immunomicroscopy (IM) 4
• Immunostaining (ISt) 3
• Immunofluorescence (Cultured Cells) (IF (cc)) 2
• Immunofluorescence (fixed cells) (IF/ICC) 2
• ChIP DNA-Sequencing (ChIP-seq) 1
• Chromatin Immunoprecipitation (ChIP) 1
• Immunoassay (IA) 1
• Immunoelectron Microscopy (IEM) 1

Specificity: The monoclonal detects wild type and recombinant GFP. This antibody does notdetect enhanced GFP.

Immunogen: GFP antibody (HRP) was raised in Mouse using The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. as the immunogen.

Clone: 215

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % thimerisol. Immunoglobulin and protease free.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for GFP

Target: GFP

Alternative Name: GFP ()

Synonyms: green fluorescent protein, gfp

Target Type: Tag