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IL-3 antibody

The Rabbit Polyclonal anti-IL-3 antibody has been validated for WB, ELISA and Inhibition. It is suitable to detect IL-3 in samples from Human.
Catalog No. ABIN289346

Quick Overview for IL-3 antibody (ABIN289346)

Target

See all IL-3 Antibodies
IL-3 (Interleukin 3 (IL-3))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This IL-3 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)
  • Purification

    Purified

    Immunogen

    IL3 antibody was raised in rabbit using highly pure recombinant human IL-3 as the immunogen.
  • Application Notes

    ELISA: 0.5-2 µg/mL, Inhibition: 0.38-0.57 µg/mL, WB: 0.1-0.2 µg/mL
    Optimal conditions should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute in sterile water at a concentration of >0.2 mg/mL.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS, pH 7.4.

    Handling Advice

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
  • Target

    IL-3 (Interleukin 3 (IL-3))

    Alternative Name

    IL3

    Background

    IL3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL3 may have additional immunoregulatory roles. Many of the IL3 activities depend upon co-stimulation with other cytokines. IL3 is species-specific, variably glycosylated cytokine.

    Molecular Weight

    15.1 kDa (predicted detection band MW)

    Pathways

    JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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