COL3 antibody

Catalog No. ABIN289411

This Rabbit Polyclonal antibody specifically detects COL3 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for COL3 antibody (ABIN289411)

Target: COL3 (Collagen, Type III (COL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-COL3 Antibody

Cross-Reactivity (Details): Collagen Type IIl 100%, Minimal cross reactivity to Type I, II, IV, V, and VI Collagens0.5mg/ml

Characteristics: Affinity purified Rabbit polyclonal Collagen Type III antibody
Bioactivity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Purification: Affinity chromatography purified

Sterility: Filtered

Immunogen: Collagen type III antibody was raised in rabbit using collagen type III from human and bovine placenta as the immunogen.

Application Details

Application Notes: ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:500-1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid in 0.125 M Na5 5 7, pH 8.0, with 0.075 NaCl, 0.005 EDTA, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for COL3

Target: COL3 (Collagen, Type III (COL3))

Alternative Name: Collagen Type III

Background: Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Collagen alpha-1(III) chain is a protein that in humans is encoded by the COL3A1 gene, which is located on chromosome 2. Collagen alpha-1(III) chain is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I collagen.