ESR2 antibody

Catalog No. ABIN289423

This Rabbit Polyclonal antibody specifically detects ESR2 in WB, IF, ICC, IHC (p), IHC (fro) and GS. It exhibits reactivity toward Rat.

Quick Overview for ESR2 antibody (ABIN289423)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Gel Shift (GS)

Product Details anti-ESR2 Antibody

Specificity: The immunogen for this antibody is completely conserved in human and marmoset ERB protein.

Cross-Reactivity: Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)

Purification: Affinity chromatography purified

Immunogen: Estrogen receptor beta antibody was raised in rabbit using a synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat estrogen receptor beta as the immunogen.

Isotype: IgG

Application Details

Application Notes: Gel Shift: 1-5 µL, ICC: 2-4 µg/mL, IF: 0.5 µg/mL, IHC-F: 1-2 µg/mL, IHC-P: 1-2 µg/mL WB: 1-2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Affinity purified IgG supplied in PBS with 1 mg/mL BSA and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen Receptor beta

Background: Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific geneactivation through direct interaction with estrogen response elements (ERE's).

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling