GLUT 13 antibody

Catalog No. ABIN289435

Product Details

Target: GLUT 13

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): This human GT3 peptide sequence is 100 % conserved in rat Glut-13/HMIT. It has not yet been cloned from other species.

Purification: Affinity chromatography purified

Immunogen: GLUT13 antibody was raised in rabbit using a 16 amino acid peptide from human GT13 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 0.5-1 µg/mL, IHC: 2-10 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS to a concentration of 1 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from Tris, pH 7.5, with 0.02 % BSA, and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References

Kavanagh Williamson, Coombes, Juszczak, Athanasopoulos, Khan, Eykyn, Srenathan, Taams, Dias Zeidler, Da Poian, Huthoff: "Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1." in: Viruses, Vol. 10, Issue 3, (2018) (PubMed).

Target Details

Target: GLUT 13

Background: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-13 or proton myo-inositol transporter (HMIT, human 629 aa, rat 618 aa, ~75-90 kDa/67 kDa protein) is highly expressed in glial cells and some neurons.