UCP3 antibody

Catalog No. ABIN289565

The Rabbit Polyclonal anti-UCP3 antibody is suitable to detect UCP3 in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for UCP3 antibody (ABIN289565)

Target: UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-UCP3 Antibody

Specificity: The Human UCP32-P peptide sequences is 100% Conserved in Long and Short form of human UCP3, 100% in Pig and Canine, 92% in Mouse, Rat and bovine UCP3.

Cross-Reactivity (Details): This human UCP3 peptide sequences is 100 % conserved in long and short form of human UCP3, 100 % in pig, and canine, 92 % in mouse, rat, and bovine UCP3. It has no significant homology with UCP1-2 or UCP4-5.

Purification: Affinity chromatography purified

Immunogen: UCP3 antibody was raised in rabbit using a 14 amino acid peptide of human UCP3 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Affinity purified IgG supplied in 100 mM Tris, pH 7.5, with 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for UCP3

Target: UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))

Alternative Name: UCP3

Background: Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and it is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Pathways: Proton Transport