SLC25A27 antibody

Catalog No. ABIN289567

This Rabbit Polyclonal antibody specifically detects SLC25A27 in WB, IF, IHC and ELISA. It exhibits reactivity toward Rat.

Quick Overview for SLC25A27 antibody (ABIN289567)

Target: SLC25A27 (Solute Carrier Family 25, Member 27 (SLC25A27))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC25A27 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-SLC25A27 Antibody

Cross-Reactivity (Details): ThIS Rat UCP4 peptide sequence is 100 % conserved in alternatively spliced isoforms a-c of rat, 85 % in human, and 71 % in mouse UCP4. UCP42-P has no significant homology with UCP1-3 or UCP-5.

Purification: Affinity chromatography purified

Immunogen: UCP4 antibody was raised in rabbit using a 14 amino acid peptide from rat UCP4 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 1 mg/mL. Do not dilute further until ready to use.

Concentration: Lot specific

Buffer: Affinity purified IgG lyophilized from 100 mM Tris, pH 7.5, with 0.02 % BSA, and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for SLC25A27

Target: SLC25A27 (Solute Carrier Family 25, Member 27 (SLC25A27))

Alternative Name: UCP4

Background: Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. UCP4 (mouse/rat 322 aa, human 323 aa, chromosome 6p11.2-q12) is expressed in both fetal and adult brain tissues.