GRK5 antibody (AA 350-400)

Catalog No. ABIN290254

This Rabbit Polyclonal antibody specifically detects GRK5 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken and Pufferfish.

Quick Overview for GRK5 antibody (AA 350-400) (ABIN290254)

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken, Pufferfish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRK5 Antibody

Binding Specificity: AA 350-400

Specificity: A portion of amino acid 350-400 of human GRK5.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acid350-400of human GRK5.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL), WB (0.5 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GRK5

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Alternative Name: GRK5

Background: Name/Gene ID: GRK5
Subfamily: GPRK
Family: Protein Kinase

Synonyms: GRK5, FP2025, GPRK5

Gene ID: 2869

UniProt: P34947

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling