TRAF5 antibody (AA 77-186)

Catalog No. ABIN290401

This Mouse Monoclonal antibody specifically detects TRAF5 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for TRAF5 antibody (AA 77-186) (ABIN290401)

Target: TRAF5 (TNF Receptor-Associated Factor 5 (TRAF5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 55A219 

Product Details anti-TRAF5 Antibody

Binding Specificity: AA 77-186

Specificity: A fusion protein corresponding to amino acids 77 to 186 of human TRAF5.

Purification: Protein G purified

Immunogen: A fusion protein corresponding to amino acids 77 to 186 of human TRAF5.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TRAF5

Target: TRAF5 (TNF Receptor-Associated Factor 5 (TRAF5))

Alternative Name: TRAF5

Background: Name/Gene ID: TRAF5

Synonyms: TRAF5, RNF84, MGC:39780, RING finger protein 84

Gene ID: 7188

UniProt: O00463

Pathways: NF-kappaB Signaling, Apoptosis