Latexin antibody (AA 2-222)

Catalog No. ABIN291079

This Sheep Polyclonal antibody specifically detects Latexin in WB, ELISA, IP and Neut. It exhibits reactivity toward Mouse.

Quick Overview for Latexin antibody (AA 2-222) (ABIN291079)

Target: Latexin (LXN)

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This Latexin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Product Details anti-Latexin Antibody

Binding Specificity: AA 2-222

Specificity: Selected for its ability to recognize mouse Latexin in direct ELISAs and Western Blots. In these formats, this antibody shows ~40 % cross-reactivity with rhLatexin.

Purification: Affinity purified

Immunogen: E. coli-derived rmLatexin (aa2-222).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP, Neut, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells. Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50 % of the proteolytic activity was restored by the antibody at 4.4 μg/mL (IC50) under conditions in which 0.1 μg/mL of the inhibitor, 0.1 μg/mL of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 Molar ratio of the antibody to the inhibitor. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Latexin

Target: Latexin (LXN)

Alternative Name: Latexin / MUM

Background: Name/Gene ID: LXN

Synonyms: LXN, ECI, MUM, Protein MUM, TCI, Latexin

Gene ID: 56925

UniProt: Q9BS40