HSP70 antibody

Catalog No. ABIN291548

The Mouse Monoclonal anti-HSP70 antibody has been validated for WB, IHC, IF, IP and FACS. It is suitable to detect HSP70 in samples from Human, Mouse, Rat, Chicken, Drosophila melanogaster, Cow, Pig, Monkey, Dog, Hamster, Sheep, Guinea Pig, Rabbit, C. elegans and Xenopus laevis.

Quick Overview for HSP70 antibody (ABIN291548)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Mouse, Rat, Chicken, Drosophila melanogaster, Cow, Pig, Monkey, Dog, Hamster, Sheep, Guinea Pig, Rabbit, C. elegans, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-HSP70 Antibody

Specificity: Recognizes 72 and 73kD proteins corresponding to the molecular mass of inducible hsp and hsc70 of SDS PAGE immunoblots. Species cross-reactivity: Human, mouse, rat, bovine, C. elegans, beluga, canine, chicken, Drosophila, fish (carp, Chinook/chum salmon, rainbow trout), guinea pig, hamster, monkey, porcine, plant (cucumber/pea), rabbit, sheep, Xenopus.

Purification: Protein G purified

Immunogen: Recombinant hsp70/hsc70 (1). Cellular Localization: Nuclear and cytoplasmic. Positive Control: BT474 or HeLa cells, breast carcinoma.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: Flo, IF, IHC, IP, WB (1 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immuno-electron Microscopy and Immunohistochemistry. Western Blot: 1 μg/mL was sufficient for colorimetric detection of Hsp/Hsc70 in 20 μg of heat shocked HeLa lysate using goat anti-mouse IgG: HRP as the secondary antibody. Positive control: BT474 or HeLa cells, breast carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 / Heat Shock Protein 70

Background: Name/Gene ID: HSP70

Synonyms: HSP70