Histone H2A antibody (AA 1-15)

Catalog No. ABIN291582

This anti-Histone H2A antibody is a Rabbit Polyclonal antibody detecting Histone H2A in WB, IHC and IHC (p). Suitable for Human and Mouse.

Quick Overview for Histone H2A antibody (AA 1-15) (ABIN291582)

Target: Histone H2A

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H2A Antibody

Binding Specificity: AA 1-15

Specificity: A 14 kD band is observed.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Bovine, Chicken (100%).

Purification: Protein G purified

Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A, GenBank no. ref|NP_066390.1|. Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Bovine, Chicken (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Histone H2A

Target: Histone H2A

NCBI Accession: NP_066390