PAX8 antibody (AA 2-14)

Catalog No. ABIN292477

This Goat Polyclonal antibody specifically detects PAX8 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit and Monkey.

Quick Overview for PAX8 antibody (AA 2-14) (ABIN292477)

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAX8 Antibody

Binding Specificity: AA 2-14

Specificity: Human PAX8. This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Elephant, Rabbit, Opossum, Platypus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide PHNSIRSGHGGLN-C from the N-terminus of human PAX8 (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Elephant, Rabbit, Opossum, Platypus (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: Name/Gene ID: PAX8

Synonyms: PAX8, Paired box 8, Paired domain gene 8, Paired box gene 8, Paired box protein Pax-8

Gene ID: 7849

NCBI Accession: NP_003457, NP_039245, NP_039246, NP_039247, NP_054698

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation