Versican antibody (AA 1344-1554)

Catalog No. ABIN292878

This anti-Versican antibody is a Goat Polyclonal antibody detecting Versican in WB, IHC, ICC and ELISA. Suitable for Human.

Quick Overview for Versican antibody (AA 1344-1554) (ABIN292878)

Target: Versican (Vcan)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Versican antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Product Details anti-Versican Antibody

Binding Specificity: AA 1344-1554

Specificity: Recognizes human Versican. Species sequence Homology: For immunizing sequence, aa1344-1554: canine (80 % ), mouse (60 %).

Purification: Immunoaffinity purified

Immunogen: Recombinant protein corresponding to aa1344-1554 from human Versican isoform V0, expressed in E. coli (P13611).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhVersican is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects Versican in Hela cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL detects Versican in cryosections of E13.5 mouse embryos. Tissue was fixed with PBS containing 4 %PFA for 20 min. at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton and 1 % BSA for 45 minutes at RT. After blocking, sections were incubated with diluted primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. Detection limit for rhVersican is ~0.1 ng/well. Optimal dilutions should be determined by each laboratory.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Target Details for Versican

Target: Versican (Vcan)

Alternative Name: VCAN / Versican

Background: Name/Gene ID: VCAN

Synonyms: VCAN, CSPG2, ERVR, GHAP, Large fibroblast proteoglycan, PG-M, Versican, WGN, Versican core protein, Versican proteoglycan, WGN1

Gene ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process