Pyruvate Kinase, Muscle (PKM) antibody

Catalog No. ABIN293067

This anti- antibody is a Goat Polyclonal antibody detecting in WB, IHC, ELISA, IHC (p) and IP. Suitable for Human and Rabbit.

Quick Overview for Pyruvate Kinase, Muscle (PKM) antibody (ABIN293067)

Target: Pyruvate Kinase, Muscle (PKM)

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details

Specificity: Pyruvate Kinase (Rabbit Muscle).

Purification: Purified IgG

Immunogen: Pyruvate kinase purified from rabbit muscle.

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:20000), IHC, IHC-P (5 μg/mL), IP, WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2 with 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details

Target: Pyruvate Kinase, Muscle (PKM)

Alternative Name: PKM / Pyruvate Kinase, Muscle

Background: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect