Renin antibody (AA 24-406)

Catalog No. ABIN293077

This Sheep Polyclonal antibody specifically detects Renin in WB, IHC, ELISA, IP and Neut. It exhibits reactivity toward Human.

Quick Overview for Renin antibody (AA 24-406) (ABIN293077)

Target: Renin (REN)

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Renin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Product Details anti-Renin Antibody

Binding Specificity: AA 24-406

Specificity: Recognizes both the pro and mature forms of human Renin. In direct ELISAs and Western Blots, this antibody shows ~20 % cross-reactivity with rmRenin-1. Species sequence homology: mouse 70 %, canine 72 %.

Purification: Affinity purified

Immunogen: NS0-derived rhRenin (aa24-406).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (3 - 10 μg/mL), IP, Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Neutralization. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 3-10 μg/mL. Immunoprecipitation: Used to immunoprecipitate rhRenin from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhRenin. The antibody was preincubated with the activated enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by the antibody at approximately 40 μg/mL (IC50) under conditions in which the enzyme was present at 2 μg/mL and the substrate concentration was 5uM. Considering the molecular masses of the enzyme (44kD) and the antibody (150 kD), IC50 was achieved at approximately 6:1 Molar ratio of the antibody to the enzyme. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 3 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Renin

Target: Renin (REN)

Alternative Name: REN / Renin 1

Background: Name/Gene ID: REN
Subfamily: Aspartic A1
Family: Protease

Synonyms: REN, Angiotensin-forming enzyme, Angiotensinogenase, HNFJ2, RENAA, Renin precursor, renal, Renin

Gene ID: 5972

UniProt: P00797

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour