UTF1 antibody (AA 44-153)

Catalog No. ABIN293228

The Goat Polyclonal anti-UTF1 antibody has been validated for WB, ELISA and ICC. It is suitable to detect UTF1 in samples from Human.

Quick Overview for UTF1 antibody (AA 44-153) (ABIN293228)

Target: UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UTF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-UTF1 Antibody

Binding Specificity: AA 44-153

Specificity: Selected for its ability to recognize UTF1 in the applications listed.

Purification: Affinity purified

Sterility: 0.2 μm filtered

Immunogen: E. coli-derived rhUTF1 (aa44-153).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile 40-50 % glycerol, PBS

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5 % trehalose.

Handling Advice: Aviod frequent freeze thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details for UTF1

Target: UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))

Alternative Name: UTF1

Background: Name/Gene ID: UTF1

Synonyms: UTF1

Gene ID: 8433

UniProt: Q5T230