CDH6 antibody (Extracellular Domain)

Catalog No. ABIN293426

The Sheep Polyclonal anti-CDH6 antibody has been validated for WB, ELISA, FACS and ICC. It is suitable to detect CDH6 in samples from Human.

Quick Overview for CDH6 antibody (Extracellular Domain) (ABIN293426)

Target: CDH6 (K-Cadherin (CDH6))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This CDH6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-CDH6 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes human Cadherin-6. In direct ELISAs and Western Blot, shows less than 5 % cross-reactivity with rhCadherin-8, rhCadherin-11, and rhCadherin-12.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rhCadherin-6 extracellular domain.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB

Usage: Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Flow Cytometry: 50 μg/mL Add 10 μL of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for CDH6

Target: CDH6 (K-Cadherin (CDH6))

Alternative Name: CDH6 / K Cadherin

Background: Name/Gene ID: CDH6
Family: Cadherin

Synonyms: CDH6, K Cadherin, K-cadherin, CAD6, Cadherin-6, Fetal kidney cadherin, KCAD, Kidney cadherin

Gene ID: 1004

UniProt: P55285

Pathways: Cell-Cell Junction Organization