LacI / Lac Repressor antibody

Catalog No. ABIN293507

The Rabbit Polyclonal anti-LacI / Lac Repressor antibody is suitable to detect LacI / Lac Repressor in samples from E. coli and Shigella sonnei. It has been validated for ELISA and WB.

Quick Overview for LacI / Lac Repressor antibody (ABIN293507)

Target: LacI / Lac Repressor

Reactivity: E. coli, Shigella sonnei

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Specificity: Recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100 % homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined

Purification: Immunoaffinity purified

Immunogen: Recombinant Lac I protein.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA (1:90000 - 1:100000), WB (1:500 - 1:2000)

Usage: This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kD in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: E. coli

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles. Do not freeze diluted antibody.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze-thaw cycles. Do not freeze diluted antibody.

Target Details

Target: LacI / Lac Repressor

Background: Name/Gene ID: LacI

Synonyms: LacI