LPIN3 antibody (AA 428-529)

Catalog No. ABIN294094

This Goat Polyclonal antibody specifically detects LPIN3 in WB, IHC, ELISA and ICC. It exhibits reactivity toward Mouse.

Quick Overview for LPIN3 antibody (AA 428-529) (ABIN294094)

Target: LPIN3 (Lipin 3 (LPIN3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This LPIN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Product Details anti-LPIN3 Antibody

Binding Specificity: AA 428-529

Specificity: Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50 % cross-reactivity with rhLipin 3 and less than 5 % cross-reactivity with rhLipin 1 and rhLipin 2.

Purification: Affinity purified

Immunogen: E. coli-derived recombinant mouse Lipin 3 (aa428-529).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4 % PFA + 14 % picric acid in PBS at 4?C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for LPIN3

Target: LPIN3 (Lipin 3 (LPIN3))

Alternative Name: LPIN3 / LIPIN-3

Background: Name/Gene ID: LPIN3

Synonyms: LPIN3, DJ620E11.2, DJ450M14.2, Lipin-3, Lipin 3, Lipin 3-like, Lipin-3-like, SMP2, DJ450M14.3, LIPN3L

Gene ID: 64900