DPPA5 antibody

Catalog No. ABIN295465

This Goat Polyclonal antibody specifically detects DPPA5 in ELISA, WB and ICC. It exhibits reactivity toward Mouse.

Quick Overview for DPPA5 antibody (ABIN295465)

Target: DPPA5 (Developmental Pluripotency Associated 5 (DPPA5))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DPPA5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-DPPA5 Antibody

Specificity: Selected for its ability to recognize mouse DPPA5 in the applications listed.

Purification: Affinity purified

Immunogen: E. coli-derived rmDPPA5.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25 % cross-reactivity with rhDPPA5 and approximately 10 % cross-reactivity with rmDPPA4. This antibody has not been optimized for Western blot detection of DPPA5 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect mouse DPPA5 in mouse embryonic stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 0.3 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for DPPA5

Target: DPPA5 (Developmental Pluripotency Associated 5 (DPPA5))

Alternative Name: DPPA5

Background: Name/Gene ID: DPPA5

Synonyms: DPPA5, ESG-1, HDPPA5, ESG1

Gene ID: 340168

UniProt: A6NC42