SKIL antibody (AA 600-684)

Catalog No. ABIN295508

The Rabbit Polyclonal anti-SKIL antibody is suitable to detect SKIL in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IHC (p).

Quick Overview for SKIL antibody (AA 600-684) (ABIN295508)

Target: SKIL (SKI-Like Oncogene (SKIL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SKIL Antibody

Binding Specificity: AA 600-684

Specificity: A portion of amino acids 600-684 of human snoN protein was used as the immunogen.

Purification: Protein G purified

Immunogen: Synthetic peptide from human SKIL.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SKIL / SNO / SnoN

Background: Name/Gene ID: SKIL

Synonyms: SKIL, Ski-related oncogene, SnoI, SnoN, SKI-like oncogene, Sno, Ski-related oncogene snoN, Ski-related protein, Ski-like protein, SnoA

Gene ID: 6498

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus