MEK3/6 (pSer189), (Ser207) antibody

Catalog No. ABIN296048

The Rabbit Polyclonal anti-MEK3/6 antibody is suitable to detect MEK3/6 in samples from Human, Mouse and Rat. It has been validated for WB, IP and ELISA.

Quick Overview for MEK3/6 (pSer189), (Ser207) antibody (ABIN296048)

Target: MEK3/6

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details

Binding Specificity: pSer189, Ser207

Specificity: Recognizes endogenous levels of human MKK3/MKK6 only when phosphorylated at Ser189/207. Does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1. Species cross-reactivity: Mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP (1:250), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:250.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: MEK3/6