SPARC antibody

Catalog No. ABIN2963678

This anti-SPARC antibody is a Mouse Monoclonal antibody detecting SPARC in WB, IHC and ELISA. Suitable for Human, Cow, Rabbit and Pig.

Quick Overview for SPARC antibody (ABIN2963678)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Cow, Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: ON1-1

Product Details anti-SPARC Antibody

Specificity: Reacts withwith human, bovine, rabbit, and porcine bone-derived and platelet derived osteonectin as well as with thrombin-stimulated platelets.

Purification: Purified

Immunogen: Bovine bone osteonectin (SPARC)

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
ELISA(1 - 10 μg/mL with immobilized antigen). WB (1 - 10 μg/mL, reducing and non-reducing conditions) IHC(1 - 10 μg/mL)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve lyophilized antibody in 50 μL of distilled water (2.0 mg/mL final concentration).

Concentration: 2 mg/mL

Buffer: PBS, pH 7.4, 1 % BSA

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

Expiry Date: 24 months

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin/SPARC

Background: SPARC is involved in various biological processes including blood coagulation and ossification.

SPARC Basement-membrane protein 40, BM-40 Osteonectin, ON Secreted protein acidic and rich in cysteine Gene name: SPARC, ON

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy