Vinculin antibody (pTyr1065)

Catalog No. ABIN2964743

This anti-Vinculin antibody is a Rabbit Polyclonal antibody detecting Vinculin in WB and IP. Suitable for Human, Mouse and Chicken.

Quick Overview for Vinculin antibody (pTyr1065) (ABIN2964743)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Vinculin Antibody

Binding Specificity: pTyr1065

Specificity: Chicken and human vinculin. Mouse vinculin (100 % homologous) has not been tested, but is expected to react.

Purification: Immunoaffinity purified

Immunogen: Produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 1065. The sequence is conserved in mouse and chicken.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IP, WB (1:1000)

Usage: Suitable for use in Western blotting applications following immunoprecipitation. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. Positive control: COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA were treated with vanadate for 24 hr.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+), 1 mg/mL BSA (IgG, protease free),  pH 7.2, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL / Vinculin

Background: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2